Human protocadherin 1 (PCDH1) ELISA Kit

The kit is for the quantitative level of PCDH1 in the sample, adopt purified Human PCDH1 to coat microtiter plate, make solid-phase antibody, then add PCDH1 to wells, Combine PCDH1 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PCDH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.