SALMONELLA ELISA Test Kit | Global Trade leader ecrobot

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SALMONELLA ELISA Test Kit

Posting date : Nov 10, 2011

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Product Description REAGEN™Salmonella ELISA Test Kit is designed for routine screening for Salmonella from cultured food and feed samples. Salmonella is a genus of gram negative bacteria belonging to the Enterobacteriaceae family. As a major contributor to foodborne illness, Salmonella is founded in poultry, meat and eggs. When Salmonella is consumed it may result in the disease known as Salmonellosis. The detection of Salmonella in food samples is often laborious and time-consuming. Several days are required to culture the bacteria from a sample followed by a final isolation and identification by biochemical and serological techniques. The REAGEN™Salmonella ELISA Test Kit provides a simple, rapid, sensitive and cost-effective enzyme immunoassay (ELISA) screening method, which enables government agencies,seafood processors,as well as quality assurance organizations,to detect positive and negative Salmonella samples to as low as 105 cells/mL level. Procedure Overview The method is based on a colorimetric ELISA assay. The monoclonal antibody against Salmonella has been coated in the plate wells. During the analysis, the sample is added. If Salmonella is present in the sample, it will bind with the antibody.A suitable broth is added to the well for rapid growth.The secondary antibody, tagged with a peroxidase enzyme, targets the Salmonella that is complexed to the primary antibody coated on the plate wells. The resulting color intensity, after addition of substrate, is related to the amount of Salmonella in the sample. Kit Contents, Storage and Shelf Life REAGEN™Salmonella ELISA Test Kit has the capacity for 96 determinations or testing of 46 samples in duplicate (assuming 4 wells for positive and negative controls). Most of the kit components should be stored at 2-8 C except for the Salmonella controls and 50X Ab#2 stock which should be stored at -20 C. Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Salmonella antibody-coated Plate 1x 96-well Plate(8 wells x 12 strips) 2-8C 1X Salmonella Negative Control 1.0 mL -20C 1X Salmonella Positive Control 1.0 mL -20C * HRP-Conjugated Antibody #2 (powder) 3 tubes -20C Antibody #2 Diluent 20 mL 2-8C 20X Wash Solution 28 mL 2-8C Stop Buffer 20 mL 2-8C TMB Substrate 12 mL 2-8C Sensitivity The kit can detect 105cells/mL