GA - PEG6 - Iodine Of NHS ester PEG Is For Targeted Drug Delivery

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GA - PEG6 - Iodine Of NHS ester PEG Is For Targeted Drug Delivery - - - - Item#: | BK02423 | CAS#: | | M.F.: | C40H70IN3O11 | M.W.: | 895.9 | Appearance: | Transparent and oil free liquid | Purity: | 95% | Storage: | -20 for Long Term, Keep cool and dry | - - - - Important Product Information GA - PEG6 - Iodine is moisture-sensitive. Store the vial of biotin reagent at 4C with desiccant. To avoid moisture condensation onto the product, equilibrate vial to room temperature before opening. As directed in the procedure, dissolve the NHS ester reagent immediately before use. The NHS moiety readily hydrolyzes and becomes non-reactive; therefore, weigh and dissolve only a small amount of the reagent at a time, and do not prepare stock solutions for storage. Discard any unused reconstituted reagent. Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction. If necessary, dialyze or desalt to exchange the protein sample into an amine-free buffer such as phosphate-buffered saline (PBS). When Pegylating proteins in solution, excess non-reacted PEG linker is easily removed by size exclusion using either desalting columns or dialysis (Additional Information). A 10mL desalting column is best suited for processing Pegylation reactions involving 1-10mg of protein in approximately 0.5-2mL. For smaller amounts of protein and/or smaller reaction volumes, both the Pegylation reaction and subsequent buffer exchange may be performed in a single Thermo Scientific Slide-A-Lyzer MINI Dialysis Unit. For larger reaction volumes than can be processed with a desalting column, either split the sample between two columns.

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Borenpharm Co., Ltd

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