MPEI CAS NO:76384-55-9

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Product
MPEI CAS NO:76384-55-9
Posting date : Nov 04, 2016
Membership
Free Member Scince Nov 04, 2016
FOB Price
95USD/MG
Min. Order Quantity
100mg
Supply Abillity
500kgs per month
Port
SHENZHEN
Payment Terms
TT or L/C
Package
1MG/bottle
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Product Detail
Company Info
 
Quick Detail
Place of Origin
China [CN]
Brand Name
Dieckmann
HS-CODE
2804-10
Package & Delivery Lead Time
Package
1MG/bottle
Delivery Lead Time
within 5 days after payment confirmed in our bank
Detailed Description
MPEI CAS NO:76384-55-9
Detailed Information:
Name: 1-methyl-3-[(E)-2-(1-methylpyridin-1-ium-4-yl)ethenyl]indole iodide
CAS NO:76384-55-9
M.F:C17H19IN2
Purity:>98%min
 
Description:
MPEI Green-Fluorescent cyanine dye (Product No.) is a cell-permeant nucleic acid stain that can selectively stain rRNA in the nucleoli. Although virtually nonfluorescent in the absence of nucleic acids,MPEI exhibits bright green fluorescence when bound to rRNA (absorption/emission maxima ~458/540 n, but only weak fluorescence when bound to ct-DNA (Figure 1). Eukaryotic cells stained with the MPEI dye show a staining pattern consistent with that of an RNA-selective probe. Maximal fluorescence is observed in the nucleoli, with faint fluorescence found throughout the cytoplasm. 
 
Application:
The RNA localization of the MPEI dye is further supported by RNase and DNase treatments:
a) Control treatment;
b) upon treatment with DNase, there is no significant loss of fluorescence;
c) upon treatment with RNase, the nucleolar and cytoplasmic intensities are significantly reduced, as compared with control cells; Because the MPEI dye is cell permeant, it is suitable for counter staining use in live cells.
For more detail information, please refer to (ACS Chem. Biol. 2015, 10, 1171−1175) http://pubs.acs.org/doi/abs/10.1021/cb500927r
 
Advantages :
 
Figure 1. Absorption and emission spectrum of MPEI without or with calf thymus DNA (ct-DNA) or 16S and 32S rRNA in T.E. buffer at pH 7.5.
 
Figure 2. The fluorometric titration of MPEI with the addition of rRNA in T.E. buffer. The RNA localization of the MPEI™ dye is further supported by RNase and DNase treatments
 
Contact :
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ShenZhen Dieckmann Technology Development Co., LTDShenZhen Address:2floor,3 building, Maluan Make Place,2-10south of Jinlong Road, Pingshan New district,ShenzhenZip code: 518172Tel: 86-755-28961872Fax: 86-755-84813563Email: sale(at)dkmchem(dot)com
 

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