Human BH4 ELISA Kit

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Human BH4 ELISA Kit
Posting date : May 20, 2013
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Free Member Scince Aug 30, 2011
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Place of Origin
United States [US]
HS-CODE
28-
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Detailed Description
The kit assay Human BH4 level in the sample,use Purified Human BH4 antibody to coat microtiter plate wells, make solid-phase antibody, then add BH4 to wells, Combined BH4 antibody which With HRP labeled goat anti-Human become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BH4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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