Total Antibiotics in Milk Test Kit

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Total Antibiotics in Milk Test Kit
Posting date : Nov 23, 2011
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Product Description REAGEN™Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits,. the unique features of the kit are:  A quick spore growth assay (results can be read in about 3 hours).  Detection of wide range of antibiotics in milk.  High sensitivity and low detection limit.  High reproducibility. Procedure Overview The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65C, these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged. Kit Contents, Storage and Shelf Life REAGEN™Total Antibiotics in Milk Test Kit has the capacity for 96 determinations for product Reference #: 1023-01 and 288 determinations for product Reference #: 1023-02, including negative and positive controls. Return any unused plates/strips/wells to the plastic bag provided in the original package and reseal them. The shelf life is 6 months when the kit is properly stored. RN 33S-01 Kit Contents Amount Storage Microtiter Plate 96-well plates (8 wells x 12 strips) 2-8C Milk Negative Control Powder 1 tube Room Temperature Milk Positive Control Powder 1 tube Room Temperature Milk Negative Control Diluent 1.5 mL 2-8C Milk Positive Control Diluent** 1.5 mL -20C Aluminum Seal 2 Sheets Room Temperature QC Certificate 1 Na RN 33S-02 Kit Contents Amount Storage Microtiter Plate 3x96-well plates (8wells x 12 strips) 2-8C Milk Negative Control Powder 3 tubes RoomTemperature Milk Positive Control Powder 3 tubes RoomTemperature Milk Negative Control Diluent 3 tubes X 1.5 mL 2-8C Milk Positive Control Diluent** 3 tubes X 1.5 mL -20C Aluminum Seal 6 Sheets RoomTemperature QC Certificate 1 Na  Upon arrival, immediately store the Milk Positive Control Diluent at -20C. See storage temperature for other components. After reconstitution of the negative and positive controls, aliquot 0.1mL into separate tubes and store in freezer at -20C. Sensitivity Antibiotic Detection Limit (ppb) MRL (ppb) Penicillin G 2 4 Ampicillin 3 4 Amoxicillin 4 4 Oxacillin 20 30 Cloxacillin 30 30 Gentamycin 30 100 Tylosin 50 50 Ceftiofur 50 100 Oxytetracycline 50 100 Tetracycline 50 100 Sulfamethoxazole 200 500

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